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Cloning and expression of an Azotobacter vinelandii mannuronan C-5-epimerase gene.

An Azotobacter vinelandii mannuronan C-5-epimerase gene was cloned in Escherichia coli. This enzyme catalyzes the Ca(2+)-dependent epimerization of D-mannuronic acid residues in alginate to the corresponding epimer L-guluronic acid. The epimerase gene was identified by screening a bacteriophage EMBL...

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Autori principali: Ertesvåg, H, Doseth, B, Larsen, B, Skjåk-Braek, G, Valla, S
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1994
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC205438/
https://ncbi.nlm.nih.gov/pubmed/8188585
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