Use of the "blue halo" assay in the identification of genes encoding exported proteins with cleavable signal peptides: cloning of a Borrelia burgdorferi plasmid gene with a signal peptide.

We have recently reported a phoA expression vector, termed pMG, which, like TnphoA, is useful in identifying genes encoding membrane-spanning sequences or signal peptides. This cloning system has been modified to facilitate the distinction of outer membrane and periplasmic alkaline phosphatase (AP)...

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Bibliografische gegevens
Hoofdauteurs: Giladi, M, Champion, C I, Haake, D A, Blanco, D R, Miller, J F, Miller, J N, Lovett, M A
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1993
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Research Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC204842/
https://ncbi.nlm.nih.gov/pubmed/8320228
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