Site-directed mutagenesis of lysine 193 in Escherichia coli isocitrate lyase by use of unique restriction enzyme site elimination.

By a newly developed double-stranded mutagenesis technique, histidine (H), glutamate (E), arginine (R) and leucine (L) have been substituted for the lysyl 193 residue (K-193) in isocitrate lyase from Escherichia coli. The substitutions for this residue, which is present in a highly conserved, cation...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Diehl, P, McFadden, B A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204513/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8385665/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.8.2263-2270.1993
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