Purification and Characterization of Thermostable Pullulanase from Bacillus stearothermophilus and Molecular Cloning and Expression of the Gene in Bacillus subtilis

A thermostable pullulanase (α-dextrin 6-glucanohydrolase [EC 3.2.1.41]) from a newly isolated Bacillus stearothermophilus strain (TRS128) was purified and characterized. The enzyme hydrolyzed (1→6)-α-d-glucosidic linkages of pullulan to produce maltotriose, and the optimum temperature was 65°C. Abou...

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Bibliografski detalji

Izdano u:	Appl Environ Microbiol
Glavni autori:	Kuriki, Takashi, Park, Jong-Hyun, Okada, Shigetaka, Imanaka, Tadayuki
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1988
Teme:	Genetics and Molecular Biology
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204393/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347785/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.11.2881-2883.1988
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