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Molecular cloning of 3-phenylcatechol dioxygenase involved in the catabolic pathway of chlorinated biphenyl from Pseudomonas putida and its expression in Escherichia coli.

Genes encoding 3-phenylcatechol dioxygenases were cloned from the chlorobiphenyl-degrading Pseudomonas putida strain OU83, using broad-host-range cosmid vector pCP13. Restriction enzyme analysis of DNA from 2,3-dioxygenase-positive chimeric cosmids showed DNA inserts ranging in size from 6.0 to 30 k...

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Bibliographic Details
Main Authors: Khan, A, Tewari, R, Walia, S
Format: Artigo
Language:Inglês
Published: 1988
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC204353/
https://ncbi.nlm.nih.gov/pubmed/3063207
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