Cloning of the Thermomonospora fusca Endoglucanase E2 Gene in Streptomyces lividans: Affinity Purification and Functional Domains of the Cloned Gene Product
Thermomonospora fusca YX grown in the presence of cellulose produces a number of β-1-4-endoglucanases, some of which bind to microcrystalline cellulose. By using a multicopy plasmid, pIJ702, a gene coding for one of these enzymes (E2) was cloned into Streptomyces lividans and then mobilized into bot...
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| Published in: | Appl Environ Microbiol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1988
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204303/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347759/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.10.2521-2526.1988 |
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