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Quantification of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens Strains in the Plant Rhizosphere by Real-Time PCR

A real-time PCR SYBR green assay was developed to quantify populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing (phlD(+)) strains of Pseudomonas fluorescens in soil and the rhizosphere. Primers were designed and PCR conditions were optimized to specifically amplify the phlD gene from four...

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Detalhes bibliográficos
Main Authors: Mavrodi, Olga V., Mavrodi, Dmitri V., Thomashow, Linda S., Weller, David M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2042083/
https://ncbi.nlm.nih.gov/pubmed/17630311
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00925-07
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