Simplified purification method for Clostridium botulinum type E toxin.

Clostridium botulinum type E toxin was purified in three chromatography steps. Toxin extracted from cells was concentrated by precipitation and dissolving in a small volume of citrate buffer. When the extract was chromatographed on DEAE-Sephadex without RNase or protamine treatment, the first protei...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Giménez, J A, Sugiyama, H
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1987
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204206/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3435146/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.12.2827-2830.1987
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