Molecular cloning of an endoglucanase gene from an alkalophilic Bacillus sp. and its expression in Escherichia coli.

One of the cellulase genes from alkalophilic Bacillus sp. strain N-4 was cloned in pBR322. A recombinant plasmid, pYBC107, expressing carboxymethyl cellulase (CMCase) was isolated, and the size of the cloned HindIII fragment was found to be 5.5 kilobases. The restriction map of pYBC107 showed a diff...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Kim, J M, Kong, I S, Yu, J H
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1987
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204168/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2827572/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.11.2656-2659.1987
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