Detection of hepatitis A virus by hybridization with single-stranded RNA probes.

An improved method of dot-blot hybridization to detect hepatitis A virus (HAV) was developed with single-stranded RNA (ssRNA) probes. Radioactive and nonradioactive ssRNA probes were generated by in vitro transcription of HAV templates inserted into the plasmid pGEM-1. 32P-labeled ssRNA probes were...

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Bibliographic Details
Main Authors: Jiang, X, Estes, M K, Metcalf, T G
Format: Artigo
Language:Inglês
Published: 1987
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC204134/
https://ncbi.nlm.nih.gov/pubmed/2447830
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