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A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis

BACKGROUND: The quality of chemically synthesized oligonucleotides falls with the length of the oligonucleotide, not least due to depurinations and premature termination during production. This limits the use of long oligonucleotides in assays where long high-quality oligonucleotides are needed (e.g...

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Autori principali: Lohmann, Jakob S, Stougaard, Magnus, Koch, Jørn
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2040145/
https://ncbi.nlm.nih.gov/pubmed/17705815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-7-49
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