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A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis

BACKGROUND: The quality of chemically synthesized oligonucleotides falls with the length of the oligonucleotide, not least due to depurinations and premature termination during production. This limits the use of long oligonucleotides in assays where long high-quality oligonucleotides are needed (e.g...

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Библиографические подробности
Главные авторы: Lohmann, Jakob S, Stougaard, Magnus, Koch, Jørn
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2007
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2040145/
https://ncbi.nlm.nih.gov/pubmed/17705815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-7-49
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