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A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis

BACKGROUND: The quality of chemically synthesized oligonucleotides falls with the length of the oligonucleotide, not least due to depurinations and premature termination during production. This limits the use of long oligonucleotides in assays where long high-quality oligonucleotides are needed (e.g...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
मुख्य लेखकों: Lohmann, Jakob S, Stougaard, Magnus, Koch, Jørn
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: BioMed Central 2007
विषय:
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC2040145/
https://ncbi.nlm.nih.gov/pubmed/17705815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-7-49
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