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Transposon Mutagenesis of Azospirillum brasilense and Azospirillum lipoferum: Physical Analysis of Tn5 and Tn5-Mob Insertion Mutants

Tn5-induced insertion mutants were generated in Azospirillum brasilense Sp7 and A. lipoferum SpBr17 by mating with Escherichia coli strains carrying suicide plasmid vectors. The sources of Tn5 were the suicide plasmids pGS9 and pSUP2021. Kanamycin-resistant Azospirillum colonies appeared from crosse...

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Detalhes bibliográficos
Main Authors: Vanstockem, Mark, Michiels, Kris, Vanderleyden, Jos, Van Gool, August P.
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC203674/
https://ncbi.nlm.nih.gov/pubmed/16347289
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