Protease-deficient Bacillus subtilis host strains for production of Staphylococcal protein A.
We constructed strains of Bacillus subtilis which produced very low levels of extracellular proteases. These strains carried insertion or deletion mutations in the subtilisin structural gene (apr) which were constructed in vitro by using the cloned gene. The methods used to construct the mutations i...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1987
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203669/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3032102/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.2.379-384.1987 |
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