Plasmid transformation of Streptomyces tendae after heat attenuation of restriction.
Streptomyces tendae ATCC 31160 produces nikkomycin, a fungicide and insecticide that inhibits chitin synthases. Exposure of S. tendae protoplasts to 50 degrees C for 30 min is required for transformation (10(2) thiostrepton-resistant transformants micrograms of DNA-1) with plasmid pIJ702 or pIJ680 f...
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| Izdano u: | Appl Environ Microbiol |
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| Glavni autor: | |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1987
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203590/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3030192/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.1.1-3.1987 |
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