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Separation of proximal tubule cells from suspensions of rat kidney cells in density gradients of Ficoll in tissue culture medium.

Rat kidneys were disaggregated with 0.25% trypsin. Cell were separated by velocity sedimentation in a previously described isokinetic gradient, by isopycnic sedimentation, and by velocity sedimentation followed by isopycnic sedimetation. In some fractions from the isokinetic gradient, 71.8+/-2.4+ of...

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Detalhes bibliográficos
Main Authors: Kreisberg, J. I., Pitts, A. M., Pretlow, T. G.
Formato: Artigo
Idioma:Inglês
Publicado em: 1977
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2032108/
https://ncbi.nlm.nih.gov/pubmed/842613
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