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High Efficiency of Plating of the Thermophilic Sulfur-Dependent Archaebacterium Sulfolobus acidocaldarius

A procedure for plating Sulfolobus acidocaldarius using Gelrite as the gelling agent is presented. The technique uses a supporting gel of 0.8% (wt/vol) Gelrite and an overlay soft gel of 0.4% (wt/vol) Gelrite, in which the colonies are grown. The plating efficiency was essentially 100%. IMAGES:

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Publicado no:Appl Environ Microbiol
Principais autores: Lindström, E. Börje, Sehlin, H. Mikael
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1989
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203208/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348063/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.11.3020-3021.1989
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