Selection of amidases with novel substrate specificities from penicillin amidase of Escherichia coli.

To obtain amidases with novel substrate specificity, the cloned gene for penicillin amidase of Escherichia coli ATCC 11105 was mutagenized and mutants were selected for the ability to hydrolyze glutaryl-(L)-leucine and provide leucine to Leu- host cells. Cells with the wild-type enzyme did not grow...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Forney, L J, Wong, D C, Ferber, D M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1989
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203120/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2690733/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.10.2550-2555.1989
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