Transformation of Bacillus polymyxa with plasmid DNA.

A plasmid transformation system was developed for Bacillus polymyxa ATCC 12321 and derivatives of this strain. The method utilizes a penicillin-treated-cell technique to facilitate uptake of the plasmid DNA. Low-frequency transformation (10(-6) per recipient cell) of plasmids pC194, pBD64, and pBC16...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Mallonee, D H, Speckman, R A
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1989
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203114/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2604393/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.10.2517-2521.1989
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