Plasmid transformation by electroporation of Leuconostoc paramesenteroides and its use in molecular cloning.

In this report, we demonstrate the utility of electroporation as an efficient method for genetic transformation of Leuconostoc paramesenteroides. We optimized several factors which determine the transformation frequency, resulting in transformation efficiencies of up to 4 x 10(3) transformants per m...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	David, S, Simons, G, De Vos, W M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1989
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202890/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2504108/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.6.1483-1489.1989
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