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DNA amplification to enhance detection of genetically engineered bacteria in environmental samples.

The polymerase chain reaction (PCR) was performed to amplify a 1.0-kilobase (kb) probe-specific region of DNA from the herbicide-degrading bacterium Pseudomonas cepacia AC1100 in order to increase the sensitivity of detecting the organism by dot-blot analysis. The 1.0-kb region was an integral porti...

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Detalles Bibliográficos
Publicado en:Appl Environ Microbiol
Principais autores: Steffan, R J, Atlas, R M
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1988
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202834/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3190225/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.9.2185-2191.1988
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