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The Use of Mild Trypsinization Conditions in the Detachment of Endothelial Cells to Promote Subsequent Endothelialization on Synthetic Surfaces

A necessary condition for endothelialization of small diameter grafts is rapid and firm adhesion of endothelial cells upon exposure to flow. To retain integrins on the cell surface, we assessed the effects of trypsin concentration, the duration of trypsin incubation, and trypsin neutralization metho...

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Detalhes bibliográficos
Main Authors: Brown, Melissa A., Wallace, Charles S., Anamelechi, Charles C., Clermont, Edward, Reichert, William M., Truskey, George A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2025691/
https://ncbi.nlm.nih.gov/pubmed/17570483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.biomaterials.2007.05.009
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