Nanopipette Delivery of Individual Molecules to Cellular Compartments for Single-Molecule Fluorescence Tracking

We have developed a new method, using a nanopipette, for controlled voltage-driven delivery of individual fluorescently labeled probe molecules to the plasma membrane which we used for single-molecule fluorescence tracking (SMT). The advantages of the method are 1), application of the probe to prede...

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Bibliografske podrobnosti
Main Authors: Bruckbauer, Andreas, James, Peter, Zhou, Dejian, Yoon, Ji Won, Excell, David, Korchev, Yuri, Jones, Roy, Klenerman, David
Format: Artigo
Jezik:Inglês
Izdano: The Biophysical Society 2007
Teme:
Membranes
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2025666/
https://ncbi.nlm.nih.gov/pubmed/17631532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.104737
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