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DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community

We developed a protocol which yields purified bacterial DNA from the soil bacterial community. The bacteria were first dispersed and separated from soil particles in the presence of polyvinylpolypyrrolidone, which removes humic acid contaminants by adsorption to this insoluble polymer. The soil bact...

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Autori principali: Holben, William E., Jansson, Janet K., Chelm, Barry K., Tiedje, James M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1988
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC202529/
https://ncbi.nlm.nih.gov/pubmed/16347582
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