A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation

An electroporation procedure for the plasmid-mediated genetic transformation of intact cells of Streptococcus cremoris and Streptococcus lactis was performed. Ten different strains were transformed. The method was simple and rapid and yielded transformant colonies in 14 to 24 h. The method was optim...

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Podrobná bibliografie

Vydáno v:	Appl Environ Microbiol
Hlavní autoři:	Powell, Ian B., Achen, Marc G., Hillier, Alan J., Davidson, Barrie E.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1988
Témata:	Genetics and Molecular Biology
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202521/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347576/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.3.655-660.1988
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