Purification of Pseudomonas putida acyl coenzyme A ligase active with a range of aliphatic and aromatic substrates.

Acyl coenzyme A (acyl-CoA) ligase (acyl-CoA synthetase [ACoAS]) from Pseudomonas putida U was purified to homogeneity (252-fold) after this bacterium was grown in a chemically defined medium containing octanoic acid as the sole carbon source. The enzyme, which has a mass of 67 kDa, showed maximal ac...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Fernández-Valverde, M, Reglero, A, Martinez-Blanco, H, Luengo, J M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202253/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8476289/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.4.1149-1154.1993
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