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Purification of Pseudomonas putida acyl coenzyme A ligase active with a range of aliphatic and aromatic substrates.

Acyl coenzyme A (acyl-CoA) ligase (acyl-CoA synthetase [ACoAS]) from Pseudomonas putida U was purified to homogeneity (252-fold) after this bacterium was grown in a chemically defined medium containing octanoic acid as the sole carbon source. The enzyme, which has a mass of 67 kDa, showed maximal ac...

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Main Authors: Fernández-Valverde, M, Reglero, A, Martinez-Blanco, H, Luengo, J M
格式: Artigo
語言:Inglês
出版: 1993
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC202253/
https://ncbi.nlm.nih.gov/pubmed/8476289
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