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Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.

We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). D...

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Publicat a:Appl Environ Microbiol
Autors principals: Muyzer, G, de Waal, E C, Uitterlinden, A G
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1993
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202176/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7683183/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.3.695-700.1993
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