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Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe.

An oligonucleotide DNA probe (VVAP) was constructed from a portion of the Vibrio vulnificus cytolysin gene (hylA) sequence and labeled with alkaline phosphatase covalently linked to the DNA. Control and environmental isolates probed with VVAP showed an exact correlation with results obtained with a...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Wright, A C, Miceli, G A, Landry, W L, Christy, J B, Watkins, W D, Morris, J G
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202140/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8434919/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.2.541-546.1993
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