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Detection of Escherichia coli in sewage and sludge by polymerase chain reaction.

A method in which the polymerase chain reaction (PCR) was used was developed to amplify either a uidA gene fragment or a 16S rRNA gene fragment from Escherichia coli in sewage and sludge. Because of interference caused by humic acidlike substances, crude DNA extracts were purified with a Sephadex G-...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Tsai, Y L, Palmer, C J, Sangermano, L R
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1993
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202112/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8434906/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.2.353-357.1993
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