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Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization.

A plasmid containing the cloned listeriolysin gene of Listeria monocytogenes was used as a probe to identify Listeria strains by DNA colony hybridization. The probe DNA was labeled with horseradish peroxidase in the presence of glutaraldehyde. After the hybridization and wash procedures, the hybrid...

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Detalles Bibliográficos
Publicado en:Appl Environ Microbiol
Autores principales: Datta, A R, Moore, M A, Wentz, B A, Lane, J
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 1993
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202069/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8439145/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.1.144-149.1993
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