Formation and Regeneration of Methanococcus voltae Protoplasts

Methanococcus voltae cells were converted into protoplasts by suspension in anaerobic 0.1 M Tris-HCl buffer containing 0.4 M sucrose and 0.05 M NaCl as osmoprotectants. Protoplast formation was monitored microscopically by observing the conversion of the typical irregularly shaped (uneven peripherie...

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Bibliografiske detaljer
Main Authors: Patel, G. B., Choquet, C. G., Nash, J. H. E., Sprott, G. D.
Format: Artigo
Sprog:Inglês
Udgivet: 1993
Fag:
Physiology and Biotechnology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC202050/
https://ncbi.nlm.nih.gov/pubmed/16348852
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