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A simple and highly sensitive radioenzymatic assay for lysophosphatidic acid quantification

The objective of the present work was to develop a simple and sensitive radioenzymatic assay to quantify lysophosphatidic acid (LPA). For that, a recombinant rat lysophosphatidic acid acyl transferase (LPAAT) produced in Escherichia coli was used. In the presence of [(14)C]oleoyl CoA, LPAAT selectiv...

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Auteurs principaux: Saulnier-Blache, Jean Sébastien, Girard, Alexia, Simon, Marie-Françoise, Lafontan, Max, Valet, Philippe
Format: Artigo
Langue:Inglês
Publié: Federation Of American Societies For Experimental Biology 2000
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2020493/
https://ncbi.nlm.nih.gov/pubmed/11108727
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