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Detection of β-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate

β-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by β-glucosidase action reacts with ferric ion to produce a black band, corresponding to the β-glucosidase, again...

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Autori principali: Kwon, Ki-Sun, Lee, Jaehoon, Kang, Hyung Gyoo, Hah, Yung Chil
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1994
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC202023/
https://ncbi.nlm.nih.gov/pubmed/16349468
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