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Detection of β-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate
β-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by β-glucosidase action reacts with ferric ion to produce a black band, corresponding to the β-glucosidase, again...
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| Main Authors: | , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1994
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC202023/ https://ncbi.nlm.nih.gov/pubmed/16349468 |
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