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High-precision mapping of protein–protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform

Understanding networks of protein–protein interactions constitutes an essential component on a path towards comprehensive description of cell function. Whereas efficient techniques are readily available for the initial identification of interacting protein partners, practical strategies are lacking...

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Detalhes bibliográficos
Main Authors: Pajunen, Maria, Turakainen, Hilkka, Poussu, Eini, Peränen, Johan, Vihinen, Mauno, Savilahti, Harri
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2018616/
https://ncbi.nlm.nih.gov/pubmed/17702760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm563
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