Electroporation of Alcaligenes eutrophus with (mega) plasmids and genomic DNA fragments.

Electroporation was used as a tool to explore the genetics of the heavy-metal-resistant strain Alcaligenes eutrophus CH34. A 12.9-kb A. eutrophus-Escherichia coli shuttle vector, pMOL850, was constructed to optimize electroporation conditions. This vector is derived from the E. coli plasmid pSUP202...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Taghavi, S, van der Lelie, D, Mergeay, M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201859/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7986037/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.10.3585-3591.1994
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