Purification and characterization of an amidase from an acrylamide-degrading Rhodococcus sp.

A constitutively expressed aliphatic amidase from a Rhodococcus sp. catalyzing acrylamide deamination was purified to electrophoretic homogeneity. The molecular weight of the native enzyme was estimated to be 360,000. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified prepa...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Nawaz, M S, Khan, A A, Seng, J E, Leakey, J E, Siitonen, P H, Cerniglia, C E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201808/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7944367/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.9.3343-3348.1994
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