Cloning of the pyruvate kinase gene (pyk) of Corynebacterium glutamicum and site-specific inactivation of pyk in a lysine-producing Corynebacterium lactofermentum strain.

The pyruvate kinase gene pyk from Corynebacterium glutamicum was cloned by applying a combination of PCR, site-specific mutagenesis, and complementation. A 126-bp DNA fragment central to the C. glutamicum pyk gene was amplified from genomic DNA by PCR with degenerate oligonucleotides as primers. The...

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Detaylı Bibliyografya

Yayımlandı:	Appl Environ Microbiol
Asıl Yazarlar:	Gubler, M, Jetten, M, Lee, S H, Sinskey, A J
Materyal Türü:	Artigo
Dil:	Inglês
Baskı/Yayın Bilgisi:	American Society for Microbiology (ASM) 1994
Konular:	Research Article
Online Erişim:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201675/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8074527/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.7.2494-2500.1994
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