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Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene.

The endoglucanase CelB isolated from culture filtrates of Streptomyces lividans IAF9 has an M(r) of 36,000. With carboxymethyl cellulose as the substrate, the Vmax and Km values are 110 IU/mg of enzyme and 1.3 mg/ml, respectively. Comparison of primary amino acid sequences classifies CelB in the H f...

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Bibliografische gegevens
Gepubliceerd in:Appl Environ Microbiol
Hoofdauteurs: Wittmann, S, Shareck, F, Kluepfel, D, Morosoli, R
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 1994
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201545/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8017952/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.5.1701-1703.1994
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