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Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR.

The suitability of a 16S rRNA-based mycoplasma group-specific PCR for the detection of mycoplasma contamination in cell cultures was investigated. A total of 104 cell cultures were tested by using microbiological culture, DNA fluorochrome staining, DNA-rRNA hybridization, and PCR techniques. A compa...

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Bibliografische gegevens
Hoofdauteurs: van Kuppeveld, F J, Johansson, K E, Galama, J M, Kissing, J, Bölske, G, van der Logt, J T, Melchers, W J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1994
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC201282/
https://ncbi.nlm.nih.gov/pubmed/7509584
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