Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR.

The suitability of a 16S rRNA-based mycoplasma group-specific PCR for the detection of mycoplasma contamination in cell cultures was investigated. A total of 104 cell cultures were tested by using microbiological culture, DNA fluorochrome staining, DNA-rRNA hybridization, and PCR techniques. A compa...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	van Kuppeveld, F J, Johansson, K E, Galama, J M, Kissing, J, Bölske, G, van der Logt, J T, Melchers, W J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1994
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201282/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7509584/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.1.149-152.1994
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