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Cloning and sequencing of the Thermoanaerobacterium saccharolyticum B6A-RI apu gene and purification and characterization of the amylopullulanase from Escherichia coli.

The amylopullulanase gene (apu) of the thermophilic anaerobic bacterium Thermoanaerobacterium saccharolyticum B6A-RI was cloned into Escherichia coli. The complete nucleotide sequence of the gene was determined. It encoded a protein consisting of 1,288 amino acids with a signal peptide of 35 amino a...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Ramesh, M V, Podkovyrov, S M, Lowe, S E, Zeikus, J G
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201274/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8117096/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.1.94-101.1994
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