Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae

Autophagy is a highly conserved, degradative process in eukaryotic cells. The rapamycin-sensitive Tor kinase complex 1 (TORC1) has a major role in regulating induction of autophagy; however, the regulatory mechanisms are not fully understood. Here, we find that the protein kinase A (PKA) and Sch9 si...

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Main Authors: Yorimitsu, Tomohiro, Zaman, Shadia, Broach, James R., Klionsky, Daniel J.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Cell Biology 2007
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1995722/
https://ncbi.nlm.nih.gov/pubmed/17699586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E07-05-0485
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spelling pubmed-19957222007-11-26 Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae Yorimitsu, Tomohiro Zaman, Shadia Broach, James R. Klionsky, Daniel J. Mol Biol Cell Articles Autophagy is a highly conserved, degradative process in eukaryotic cells. The rapamycin-sensitive Tor kinase complex 1 (TORC1) has a major role in regulating induction of autophagy; however, the regulatory mechanisms are not fully understood. Here, we find that the protein kinase A (PKA) and Sch9 signaling pathways regulate autophagy cooperatively in yeast. Autophagy is induced in cells when PKA and Sch9 are simultaneously inactivated. Mutant alleles of these kinases bearing a mutation that confers sensitivity to the ATP-analogue inhibitor C3-1′-naphthyl-methyl PP1 revealed that autophagy was induced independently of effects on Tor kinase. The PKA–Sch9-mediated autophagy depends on the autophagy-related 1 kinase complex, which is also essential for TORC1-regulated autophagy, the transcription factors Msn2/4, and the Rim15 kinase. The present results suggest that autophagy is controlled by the signals from at least three partly separate nutrient-sensing pathways that include PKA, Sch9, and TORC1. The American Society for Cell Biology 2007-10 /pmc/articles/PMC1995722/ /pubmed/17699586 http://dx.doi.org/10.1091/mbc.E07-05-0485 Text en © 2007 by The American Society for Cell Biology
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Articles
spellingShingle Articles
Yorimitsu, Tomohiro
Zaman, Shadia
Broach, James R.
Klionsky, Daniel J.
Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae
description Autophagy is a highly conserved, degradative process in eukaryotic cells. The rapamycin-sensitive Tor kinase complex 1 (TORC1) has a major role in regulating induction of autophagy; however, the regulatory mechanisms are not fully understood. Here, we find that the protein kinase A (PKA) and Sch9 signaling pathways regulate autophagy cooperatively in yeast. Autophagy is induced in cells when PKA and Sch9 are simultaneously inactivated. Mutant alleles of these kinases bearing a mutation that confers sensitivity to the ATP-analogue inhibitor C3-1′-naphthyl-methyl PP1 revealed that autophagy was induced independently of effects on Tor kinase. The PKA–Sch9-mediated autophagy depends on the autophagy-related 1 kinase complex, which is also essential for TORC1-regulated autophagy, the transcription factors Msn2/4, and the Rim15 kinase. The present results suggest that autophagy is controlled by the signals from at least three partly separate nutrient-sensing pathways that include PKA, Sch9, and TORC1.
author Yorimitsu, Tomohiro
Zaman, Shadia
Broach, James R.
Klionsky, Daniel J.
author_facet Yorimitsu, Tomohiro
Zaman, Shadia
Broach, James R.
Klionsky, Daniel J.
author_sort Yorimitsu, Tomohiro
title Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae
title_short Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae
title_full Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae
title_fullStr Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae
title_full_unstemmed Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae
title_sort protein kinase a and sch9 cooperatively regulate induction of autophagy in saccharomyces cerevisiae
publisher The American Society for Cell Biology
publishDate 2007
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1995722/
https://ncbi.nlm.nih.gov/pubmed/17699586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E07-05-0485
_version_ 1748578875745501184

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