Solubilization and characterization of guanine nucleotide-sensitive muscarinic agonist binding sites from rat myocardium.

Muscarinic receptors from rat myocardial membranes may be solubilized by digitonin in good yield at low temperatures in the presence of Mg2+. Under these conditions, up to 60% of the soluble receptors show high affinity binding for the potent agonist [3H]-oxotremorine-M (KA = 10(9)M-1), which is inh...

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Bibliographic Details
Main Authors: Berrie, C. P., Birdsall, N. J., Hulme, E. C., Keen, M., Stockton, J. M.
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 1984
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1986921/
https://ncbi.nlm.nih.gov/pubmed/6478115
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