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High-resolution functional mapping of a cloned gene by genetic footprinting

We describe an efficient method for introducing and analyzing a comprehensive set of mutations in a cloned gene to map its functional organization. The technique, genetic footprinting, uses a retroviral integrase to generate a comprehensive library of mutants, each of which bears a single insertion...

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書目詳細資料
Main Authors: Singh, Ila R., Crowley, Rachel Adams, Brown, Patrick O.
格式: Artigo
語言:Inglês
出版: The National Academy of Sciences of the USA 1997
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC19786/
https://ncbi.nlm.nih.gov/pubmed/9037048
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