Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana cyclophilin 38 (AtCyp38)

AtCyp38 is one of the highly divergent multidomain cyclophilins from Arabidopsis thaliana. A recombinant form of AtCyp38 (residues 83–437) was expressed in Escherichia coli and purified to homogeneity. The protein was crystallized using the vapour-batch technique with PEG 6000 and t-butanol as preci...

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Autores principales: Vasudevan, Dileep, Gopalan, Gayathri, He, Zengyong, Luan, Sheng, Swaminathan, Kunchithapadam
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2005
Materias:
Crystallization Communications
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1978155/
https://ncbi.nlm.nih.gov/pubmed/16511242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105037681
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