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Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallization
Two proposals recommend substitution of surface lysine residues as a means to improve the quality of protein crystals. In proposal I, substitution of lysine by alanine has been suggested to improve crystallization by reducing the entropic cost of ordering flexible side chains at crystal contacts. In...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1978152/ https://ncbi.nlm.nih.gov/pubmed/16511237 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105036559 |
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