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Overexpression, purification and crystallization of tyrosyl-tRNA synthetase from the hyperthermophilic archaeon Aeropyrum pernix K1

Hyperthermophilic archaeal tyrosyl-tRNA synthetase from Aeropyrum pernix K1 was cloned and overexpressed in Escherichia coli. The expressed protein was purified by Cibacron Blue affinity chromatography following heat treatment at 363 K. Crystals suitable for X-ray diffraction studies were obtained u...

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Main Authors: Iwaki, Jun, Suzuki, Ryuichiro, Fujimoto, Zui, Momma, Mitsuru, Kuno, Atsushi, Hasegawa, Tsunemi
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2005
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1978129/
https://ncbi.nlm.nih.gov/pubmed/16511219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105033245
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