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Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans
CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li(2)SO(4), 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, wi...
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| Main Authors: | , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
International Union of Crystallography
2005
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1978110/ https://ncbi.nlm.nih.gov/pubmed/16511169 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910502316X |
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