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Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li(2)SO(4), 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, wi...

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Hlavní autoři: Tricot, Catherine, van Aelst, Sebastien, Wattiez, Ruddy, Mergeay, Max, Stalon, Victor, Wouters, Johan
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1978110/
https://ncbi.nlm.nih.gov/pubmed/16511169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910502316X
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