Isolation of Microarray-Grade Total RNA, MicroRNA, and DNA from a Single PAXgene Blood RNA Tube

We have developed a procedure for isolation of microRNA and genomic DNA in addition to total RNA from whole blood stabilized in PAXgene Blood RNA tubes. The procedure is based on automatic extraction on a BioRobot MDx and includes isolation of DNA from a fraction of the stabilized blood and recovery...

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Hlavní autoři: Kruhøffer, Mogens, Dyrskjøt, Lars, Voss, Thorsten, Lindberg, Raija L.P., Wyrich, Ralf, Thykjaer, Thomas, Orntoft, Torben F.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Investigative Pathology 2007
Témata:
Technical Advance
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1975097/
https://ncbi.nlm.nih.gov/pubmed/17690207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2007.060175
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